Biuret's solution shows a color change to which color in the presence of protein?

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Study for the PLTW Biomedical Science EOC Test. Engage with flashcards and multiple-choice questions, each with detailed explanations. Excel in your exam!

Multiple Choice

Biuret's solution shows a color change to which color in the presence of protein?

Explanation:
Biuret testing detects peptide bonds in proteins. In strongly alkaline conditions, copper(II) ions from the reagent form a complex with these peptide bonds, producing a purple–violet color. The appearance of this violet/purple color indicates protein is present (the reagent alone is blue; without protein it stays blue). So the color change in the presence of protein is violet or purple.

Biuret testing detects peptide bonds in proteins. In strongly alkaline conditions, copper(II) ions from the reagent form a complex with these peptide bonds, producing a purple–violet color. The appearance of this violet/purple color indicates protein is present (the reagent alone is blue; without protein it stays blue). So the color change in the presence of protein is violet or purple.

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